Effect of recombinant lactobacillus expressing canine GM-CSF on immune function in dogs.
Identifieur interne : 000D77 ( Main/Exploration ); précédent : 000D76; suivant : 000D78Effect of recombinant lactobacillus expressing canine GM-CSF on immune function in dogs.
Auteurs : Jin Young Chung [Corée du Sud] ; Eui Jae Sung ; Chun Gyu Cho ; Kyoung Won Seo ; Jong-Soo Lee ; Dong Ha Bhang ; Hee Woo Lee ; Cheol Yong Hwang ; Wan Kyu Lee ; Hwa Young Youn ; Chul Joong KimSource :
- Journal of microbiology and biotechnology [ 1017-7825 ] ; 2009.
Descripteurs français
- KwdFr :
- Administration par voie orale, Animaux, Chiens, Coronavirus canin (immunologie), Données de séquences moléculaires, Facteur de stimulation des colonies de granulocytes et de macrophages (biosynthèse), Facteur de stimulation des colonies de granulocytes et de macrophages (immunologie), Immunoglobuline A (sang), Immunoglobuline G (sang), Infections à coronavirus (), Infections à coronavirus (immunologie), Infections à coronavirus (médecine vétérinaire), Infections à coronavirus (métabolisme), Lactobacillus casei (immunologie), Lactobacillus casei (métabolisme), Maladies des chiens (), Maladies des chiens (immunologie), Maladies des chiens (métabolisme), Maladies des chiens (virologie), Monocytes (), Poids du corps, Probiotiques (administration et posologie), Production d'anticorps (), Protéines recombinantes (biosynthèse), Protéines recombinantes (immunologie), Séquence d'acides aminés, Séquence nucléotidique, Vaccins antiviraux (immunologie), Vaccins antiviraux (pharmacologie).
- MESH :
- administration et posologie : Probiotiques.
- biosynthèse : Facteur de stimulation des colonies de granulocytes et de macrophages, Protéines recombinantes.
- immunologie : Coronavirus canin, Facteur de stimulation des colonies de granulocytes et de macrophages, Infections à coronavirus, Lactobacillus casei, Maladies des chiens, Protéines recombinantes, Vaccins antiviraux.
- médecine vétérinaire : Infections à coronavirus.
- métabolisme : Infections à coronavirus, Lactobacillus casei, Maladies des chiens.
- pharmacologie : Vaccins antiviraux.
- sang : Immunoglobuline A, Immunoglobuline G.
- virologie : Maladies des chiens.
- Administration par voie orale, Animaux, Chiens, Données de séquences moléculaires, Infections à coronavirus, Maladies des chiens, Monocytes, Poids du corps, Production d'anticorps, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Administration, Oral, Amino Acid Sequence, Animals, Antibody Formation (drug effects), Base Sequence, Body Weight, Coronavirus Infections (immunology), Coronavirus Infections (metabolism), Coronavirus Infections (prevention & control), Coronavirus Infections (veterinary), Coronavirus, Canine (immunology), Dog Diseases (immunology), Dog Diseases (metabolism), Dog Diseases (prevention & control), Dog Diseases (virology), Dogs, Granulocyte-Macrophage Colony-Stimulating Factor (biosynthesis), Granulocyte-Macrophage Colony-Stimulating Factor (immunology), Immunoglobulin A (blood), Immunoglobulin G (blood), Lactobacillus casei (immunology), Lactobacillus casei (metabolism), Molecular Sequence Data, Monocytes (drug effects), Probiotics (administration & dosage), Recombinant Proteins (biosynthesis), Recombinant Proteins (immunology), Viral Vaccines (immunology), Viral Vaccines (pharmacology).
- MESH :
- chemical , biosynthesis : Granulocyte-Macrophage Colony-Stimulating Factor, Recombinant Proteins.
- chemical , blood : Immunoglobulin A, Immunoglobulin G.
- administration & dosage : Probiotics.
- drug effects : Antibody Formation, Monocytes.
- immunology : Coronavirus Infections, Coronavirus, Canine, Dog Diseases, Granulocyte-Macrophage Colony-Stimulating Factor, Lactobacillus casei, Recombinant Proteins, Viral Vaccines.
- metabolism : Coronavirus Infections, Dog Diseases, Lactobacillus casei.
- chemical , pharmacology : Viral Vaccines.
- prevention & control : Coronavirus Infections, Dog Diseases.
- veterinary : Coronavirus Infections.
- virology : Dog Diseases.
- Administration, Oral, Amino Acid Sequence, Animals, Base Sequence, Body Weight, Dogs, Molecular Sequence Data.
Abstract
Many Lactobacillus strains have been promoted as good probiotics for the prevention and treatment of diseases. We engineered recombinant Lactobacillus casei, producing biologically active canine granulocyte macrophage colony stimulating factor (cGM-CSF), and investigated its possibility as a good probiotic agent for dogs. Expression of the cGM-CSF protein in the recombinant Lactobacillus was confirmed by SDS-PAGE and Western blotting methods. For the in vivo study, 18 Beagle puppies of 7 weeks of age were divided into three groups; the control group was fed only on a regular diet and the two treatment groups were fed on a diet supplemented with either 1 x 10(9) colony forming units (CFU)/day of L. casei or L. casei expressing cGM-CSF protein for 7 weeks. Body weight was measured, and fecal and blood samples were collected from the dogs during the experiment for the measurement of hematology, fecal immunoglobulin (Ig)A and IgG, circulating IgA and IgG, and canine corona virus (CCV)-specific IgG. There were no differences in body weights among the groups, but monocyte counts in hematology and serum IgA were higher in the group receiving L. casei expressing cGMCSF than in the other two groups. After the administration of CCV vaccine, CCV-specific IgG in serum increased more in the group supplemented with L. casei expressing cGM-CSF than the other two groups. This study shows that a dietary L. casei expressing cGM-CSF enhances specific immune functions at both the mucosal and systemic levels in puppies.
DOI: 10.4014/jmb.0904.4010
PubMed: 19996694
Affiliations:
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Le document en format XML
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effects)</term><term>Probiotics (administration & dosage)</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (immunology)</term><term>Viral Vaccines (immunology)</term><term>Viral Vaccines (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Administration par voie orale</term><term>Animaux</term><term>Chiens</term><term>Coronavirus canin (immunologie)</term><term>Données de séquences moléculaires</term><term>Facteur de stimulation des colonies de granulocytes et de macrophages (biosynthèse)</term><term>Facteur de stimulation des colonies de granulocytes et de macrophages (immunologie)</term><term>Immunoglobuline A (sang)</term><term>Immunoglobuline G (sang)</term><term>Infections à coronavirus ()</term><term>Infections à coronavirus (immunologie)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Infections à coronavirus (métabolisme)</term><term>Lactobacillus casei (immunologie)</term><term>Lactobacillus casei 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Diseases</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Immunoglobuline A</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies des chiens</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Dog Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Administration, Oral</term><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Body Weight</term><term>Dogs</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Administration par voie orale</term><term>Animaux</term><term>Chiens</term><term>Données de séquences moléculaires</term><term>Infections à coronavirus</term><term>Maladies des chiens</term><term>Monocytes</term><term>Poids du corps</term><term>Production d'anticorps</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Many Lactobacillus strains have been promoted as good probiotics for the prevention and treatment of diseases. We engineered recombinant Lactobacillus casei, producing biologically active canine granulocyte macrophage colony stimulating factor (cGM-CSF), and investigated its possibility as a good probiotic agent for dogs. Expression of the cGM-CSF protein in the recombinant Lactobacillus was confirmed by SDS-PAGE and Western blotting methods. For the in vivo study, 18 Beagle puppies of 7 weeks of age were divided into three groups; the control group was fed only on a regular diet and the two treatment groups were fed on a diet supplemented with either 1 x 10(9) colony forming units (CFU)/day of L. casei or L. casei expressing cGM-CSF protein for 7 weeks. Body weight was measured, and fecal and blood samples were collected from the dogs during the experiment for the measurement of hematology, fecal immunoglobulin (Ig)A and IgG, circulating IgA and IgG, and canine corona virus (CCV)-specific IgG. There were no differences in body weights among the groups, but monocyte counts in hematology and serum IgA were higher in the group receiving L. casei expressing cGMCSF than in the other two groups. After the administration of CCV vaccine, CCV-specific IgG in serum increased more in the group supplemented with L. casei expressing cGM-CSF than the other two groups. This study shows that a dietary L. casei expressing cGM-CSF enhances specific immune functions at both the mucosal and systemic levels in puppies.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><noCountry><name sortKey="Bhang, Dong Ha" sort="Bhang, Dong Ha" uniqKey="Bhang D" first="Dong Ha" last="Bhang">Dong Ha Bhang</name><name sortKey="Cho, Chun Gyu" sort="Cho, Chun Gyu" uniqKey="Cho C" first="Chun Gyu" last="Cho">Chun Gyu Cho</name><name sortKey="Hwang, Cheol Yong" sort="Hwang, Cheol Yong" uniqKey="Hwang C" first="Cheol Yong" last="Hwang">Cheol Yong Hwang</name><name sortKey="Kim, Chul Joong" sort="Kim, Chul Joong" uniqKey="Kim C" first="Chul Joong" last="Kim">Chul Joong Kim</name><name sortKey="Lee, Hee Woo" sort="Lee, Hee Woo" uniqKey="Lee H" first="Hee Woo" last="Lee">Hee Woo Lee</name><name sortKey="Lee, Jong Soo" sort="Lee, Jong Soo" uniqKey="Lee J" first="Jong-Soo" last="Lee">Jong-Soo Lee</name><name sortKey="Lee, Wan Kyu" sort="Lee, Wan Kyu" uniqKey="Lee W" first="Wan Kyu" last="Lee">Wan Kyu Lee</name><name sortKey="Seo, Kyoung Won" sort="Seo, Kyoung Won" uniqKey="Seo K" first="Kyoung Won" last="Seo">Kyoung Won Seo</name><name sortKey="Sung, Eui Jae" sort="Sung, Eui Jae" uniqKey="Sung E" first="Eui Jae" last="Sung">Eui Jae Sung</name><name sortKey="Youn, Hwa Young" sort="Youn, Hwa Young" uniqKey="Youn H" first="Hwa Young" last="Youn">Hwa Young Youn</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Chung, Jin Young" sort="Chung, Jin Young" uniqKey="Chung J" first="Jin Young" last="Chung">Jin Young Chung</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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